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Aflatoxins are toxic metabolites produced by a variety of molds such as Aspergillus flavus and Aspergillus parasiticus. They are carcinogenics and can be present in grains, nuts, cottonseed and other commodities associated with human food or animal feeds. Crops may be contaminated by one or more of the four following sub-types of aflatoxin: B1, B2, G1 and G2. Aflatoxin B1 is the most toxic and frequently detected form. The other types present a significant danger if the concentration is at a high level. Aflatoxin have been implicated in human health disorders including hepatocellular carcinoma, aflatoxicosis, Reye’s syndrome and cronic hepatitis. Animals are exposed to aflatoxins by consumption of feeds that are contaminated by aflatoxin producing fungal strains during growth, harvest or storage. When cows are fed contaminated feed, aflatoxin B1 is converted by hydroxylation to aflatoxin M1, which is subsequently secreted in the milk of lactating cows. Aflatoxin M1 is quite stable towards the normal milk processing method such as pasteurization and if present in raw milk, it may persist into final products for human consumption. Most controlling government agencies worldwide have regulations regarding the amount of aflatoxin M1 in milk and milk products. In the EU the limit for the presence of M1 in milk and reconstituted milk powers has been set at 50 ppt.
EuroClone AFLATOXIN M1 ELISA KIT is a competitive enzyme immunoassay for the quantitative detection of Aflatoxin M1 in milk, milk powder and cheese.
NOTES, STABILITY AND STORAGE • The kits should be stored at 2-8°C and used before the expiry date printed on the label on the box. • Do not freeze. • The standard curve should be discarded after use. • Diluted Washing Buffer should not be stored for more than 8 hours and must be discarded after use. • Once open, if properly stored (resealed in dry atmosphere at 2-8°C), the microwell plate can be maintained for several months. • Do not interchange individual reagents between kits of different lot numbers. • Do not dilute reagents as this will result in loss of sensitivity and poor performance. • Do not return unused reagents back into their original bottles. The assay procedures details volumes required.