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WonderTaq is a recombinant thermostable DNA polymerase.
"Wonder Taq has exonuclease activity 5 '-> 3' exonuclease activity and has no 3 '-> 5'.
* WonderTaq DNA polymerase 1000U (concentration: 5U / uL))
* Reaction Buffer 5X (6 x 1.5ml): contains 5 mM dNTPs, MgCl2 and enhancers.
The standard reaction conditions (in 50 ul) are the following:
10 ul 5X Wonder Taq Reaction Buffer
1 ul primer fwd 20 uM
1 ul primer rev 20 uM
DNA template (see variable manual for suggestions)
0.25-1 ul Wonder Taq (equal to 1.25 to 5 units)
Definition of a unit:
* A 'unit is the amount of enzyme that during the reaction incorporates 10 nmoles of dNTPs (Nucleotides) in the acid form is not soluble in the conditions listed as "standard" at 72 ° C for 30 minutes.
* Storage: -20 ° C
Shelf life: the date indicated on the package
Product intended for use Lab-Research, does not fall between the medical devices and diagnostics in vitro, it is not subject to Directives 93/42 / EEC and 98/79 / EEC.