Recombinant Trypsin-EDTA

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Recombinant Trypsin-EDTA 1X in PBS w/o Calcium w/o Magnesium w/o Phenol Red

100 ml

Recombinant Trypsin-EDTA 1x in PBS w/o Ca, w/o Mg and W/o Phenol red

EuroClone recombinant Trypsin is an highly pure and genetically engineered protein expressed in E Coli.
As such it is totally animal free.
Recombinant Trypsin is a cell-dissociation enzyme that replaces porcine trypsin.
Free from contaminating enzymes like chymotrypsin as well as protease inhibitors.
Recombinant Trypsin eliminates the risk of viruses, or other potential adventitious agents found in animal derived components.
It is highly stable with a high purity (95%). It is widely used in cell culture applications.
Recombinant Trypsin is ideal for dissociating attachment-dependent cell lines in both serum-containing and serum-free conditions, and can be directly substituted for trypsin without protocol changes
The solution contains EDTA, a chelating agent, which enhances the enzymatic activity by removing calcium and magnesium ions.
These ions obscure the peptide bonds on which trypsin acts as well as enhancing cell to cell adhesion.

Enzyme activity >= 1250 USP units /mg of powder

Storage and Shelf life:
Sterility : Sterile filtered
State : Frozen
Storage : -20°C
If properly stored shelf life is: 24 months

For research use only